![]() TXNIP expression was associated with tumor mutational burden, microsatellite instability, mismatch repair genes, tumor infiltrating immune cell abundance as well as cancer-associated fibroblasts. TXNIP is lowly expressed in most cancers, and distinct associations exist between TXNIP expression and the prognosis of tumor patients. We thus first explored the potential roles of TXNIP across thirty-three tumors mainly based on The Cancer Genome Atlas and Gene Expression Omnibus datasets. The critical role of thioredoxin-interacting protein ( TXNIP ) in cellular sulfhydryl redox homeostasis and inflammasome activation is already widely known, however, no pan-cancer analysis is currently available. All data represent the average of two to six individual transfection experiments performed in duplicate. The GAL4-tk-Luc reporter (100 ng) was cotransfected in 293T cells along with a GAL4-RD2 (PLZF200-300) vector and an expression vector for wild-type ETO or ETO (1-322). (D) ETO enhances repression by the second repression domain of PLZF. Lanes: 6, GAL-POZ/BTB alone 7, GAL4-POZ/BTB plus ETO 8, GAL4-POZ/ BTB plus ETO (HR,Ner,MYND) 9, GAL4-POZ/BTB plus ETO (1-322) 10, GAL4-POZ/BTB plus ETO (1-322) plus wild-type ETO. In lanes 6 to 10, the GAL4-tk-Luc reporter (100 ng) was cotransfected with a vector encoding GAL4-POZ/BTB and wild-type or mutant ETO. Lanes: 1, PLZF 2, PLZF plus ETO 3, PLZF plus ETO (HR,Ner,MYND) 4, PLZF plus ETO (1-322) 5, PLZF plus ETO (1-322) plus wild-type ETO. In lanes 1 to 5, wild-type and or mutant ETO expression vectors (400 ng) were cotransfected into 293T cells along with the PLZF expression vector and I元R-tk-Luc reporter (100 ng). (C) C-terminal domains of ETO are required to enhance repression by PLZF. Repression of the GAL4-tk-Luc reporter was measured relative to expression of luciferase directed by the reporter in the presence of GAL4(1-147). 293T cells were cotransfected in duplicate with the GAL4-tk-Luc reporter (100 ng), 400 ng of the GAL4(1-147) or GAL4-POZ/BTB expression vector, and increasing amounts of ETO (100, 400, and 800 ng, respectively). (B) ETO potentiates transcriptional repression by the PLZF POZ/BTB domain. In lanes 5 to 8, the same reporter vector was coexpressed along with wild-type or mutant PLZF expression vector and 400 ng of ETO expression vector. ![]() In lanes 1 to 4, 293T cells were cotransfected with the I元R-tk-Luc reporter (100 ng) and 400 ng of the pCDNA expression vector or pCDNA vector containing sequences encoding wild-type PLZF, PLZF-POZ/BTB, or PLZF-RD2. (A) The presence of both PLZF repression domains is required for functional interaction with ETO. Identification of sequences with PLZF and ETO required for functional interaction.
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